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mouse anti havcr kim 1  (R&D Systems)


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    Structured Review

    R&D Systems mouse anti havcr kim 1
    Mouse Anti Havcr Kim 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kim+1+antibody/Mouse+TIM-1%2FKIM-1%2FHAVCR+Antibody/pm41981279-218-9-15
    Average 96 stars, based on 297 article reviews
    mouse anti havcr kim 1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Superiority of intranasal over systemic administration of bioengineered soluble ACE2 for survival and brain protection against SARS-CoV-2 infection
    Article Snippet: For immunofluorescence staining studies of the brain, an IBA1 (Abcam, ab178846) and GFAP (Abcam, ab4674) antibody were used. .. For staining studies of the kidney, an Anti Lipocain-2/NGAL antibody (Abcam, ab216462) and a KIM-1 antibody (R&D systems AF1817, a gift from Dr. Joseph Bonventre) were used. ..

    Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site.
    Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown.

    Article Title: Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury
    Article Snippet: .. To visualize kidney injury molecule-1 (KMI-1) expression by IHC staining, sections were incubated with KIM-1 antibody (R&D Systems) and biotinylated α-rabbit secondary antibody (Vector Lab) for 45 min at room temperature. ..

    Article Title: Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury.
    Article Snippet: .. To visualize kidney injury molecule-1 (KMI-1) expression by IHC staining, sections were incubated with KIM-1 antibody (R&D Systems) and biotinylated α-rabbit secondary antibody (Vector Lab) for 45 min at room temperature. ..

    Concentration Assay:

    Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site.
    Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown.

    Expressing:

    Article Title: Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury
    Article Snippet: .. To visualize kidney injury molecule-1 (KMI-1) expression by IHC staining, sections were incubated with KIM-1 antibody (R&D Systems) and biotinylated α-rabbit secondary antibody (Vector Lab) for 45 min at room temperature. ..

    Article Title: Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury.
    Article Snippet: .. To visualize kidney injury molecule-1 (KMI-1) expression by IHC staining, sections were incubated with KIM-1 antibody (R&D Systems) and biotinylated α-rabbit secondary antibody (Vector Lab) for 45 min at room temperature. ..

    Immunohistochemistry:

    Article Title: Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury
    Article Snippet: .. To visualize kidney injury molecule-1 (KMI-1) expression by IHC staining, sections were incubated with KIM-1 antibody (R&D Systems) and biotinylated α-rabbit secondary antibody (Vector Lab) for 45 min at room temperature. ..

    Article Title: Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury.
    Article Snippet: .. To visualize kidney injury molecule-1 (KMI-1) expression by IHC staining, sections were incubated with KIM-1 antibody (R&D Systems) and biotinylated α-rabbit secondary antibody (Vector Lab) for 45 min at room temperature. ..

    Incubation:

    Article Title: Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury
    Article Snippet: .. To visualize kidney injury molecule-1 (KMI-1) expression by IHC staining, sections were incubated with KIM-1 antibody (R&D Systems) and biotinylated α-rabbit secondary antibody (Vector Lab) for 45 min at room temperature. ..

    Article Title: Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury.
    Article Snippet: .. To visualize kidney injury molecule-1 (KMI-1) expression by IHC staining, sections were incubated with KIM-1 antibody (R&D Systems) and biotinylated α-rabbit secondary antibody (Vector Lab) for 45 min at room temperature. ..

    Article Title: Modulation of Tetrachloroethylene-Associated Kidney Effects by Nonalcoholic Fatty Liver or Steatohepatitis in Male C57BL/6J Mice
    Article Snippet: .. Endogenous peroxidase activity was blocked using a Peroxidase Blocking Reagent (Dako) for 5 min. After washing with PBS-T, slides were incubated for 10 min at room temperature with a goat anti KIM-1 antibody (1 μg/ml; AF1817, Lot KCA0415031; R&D systems, Minneapolis, Minnesota) prepared in an Antibody Diluent (Dako). .. After washing with PBS-T, slides were incubated with rabbit anti-goat HRP-conjugated secondary antibody (1:200 dilution; 10 min at room temperature), washed with PBS-T again, and then incubated with 3,3′-diaminobenzidine reagent (Dako) for 8 min at room temperature.

    Activity Assay:

    Article Title: Modulation of Tetrachloroethylene-Associated Kidney Effects by Nonalcoholic Fatty Liver or Steatohepatitis in Male C57BL/6J Mice
    Article Snippet: .. Endogenous peroxidase activity was blocked using a Peroxidase Blocking Reagent (Dako) for 5 min. After washing with PBS-T, slides were incubated for 10 min at room temperature with a goat anti KIM-1 antibody (1 μg/ml; AF1817, Lot KCA0415031; R&D systems, Minneapolis, Minnesota) prepared in an Antibody Diluent (Dako). .. After washing with PBS-T, slides were incubated with rabbit anti-goat HRP-conjugated secondary antibody (1:200 dilution; 10 min at room temperature), washed with PBS-T again, and then incubated with 3,3′-diaminobenzidine reagent (Dako) for 8 min at room temperature.

    Blocking Assay:

    Article Title: Modulation of Tetrachloroethylene-Associated Kidney Effects by Nonalcoholic Fatty Liver or Steatohepatitis in Male C57BL/6J Mice
    Article Snippet: .. Endogenous peroxidase activity was blocked using a Peroxidase Blocking Reagent (Dako) for 5 min. After washing with PBS-T, slides were incubated for 10 min at room temperature with a goat anti KIM-1 antibody (1 μg/ml; AF1817, Lot KCA0415031; R&D systems, Minneapolis, Minnesota) prepared in an Antibody Diluent (Dako). .. After washing with PBS-T, slides were incubated with rabbit anti-goat HRP-conjugated secondary antibody (1:200 dilution; 10 min at room temperature), washed with PBS-T again, and then incubated with 3,3′-diaminobenzidine reagent (Dako) for 8 min at room temperature.



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    R&D Systems mouse anti havcr kim 1
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    R&D Systems primary antibody against kim 1
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    Image Search Results


    ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) Serum KIM-1 levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.

    Journal: Biomedicines

    Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

    doi: 10.3390/biomedicines14092127

    Figure Lengend Snippet: ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) Serum KIM-1 levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.

    Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

    Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Analysis, Western Blot, Control

    ECH relieves cisplatin-induced cell damage in vitro. ( A ) HK-2 cell viability treated after treatment with different concentrations of ECH. ( B ) HK-2 cell viability under cisplatin stimulation with gradient ECH intervention. ( C ) Representative PI staining images of HK-2 cells and quantitative analysis. ( D ) Western blot and quantitative analysis of KIM-1 and NGAL proteins in HK-2 cells. ( E – H ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in HK-2 cells. ns, no significancant; * p < 0.05, ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group.

    Journal: Biomedicines

    Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

    doi: 10.3390/biomedicines14092127

    Figure Lengend Snippet: ECH relieves cisplatin-induced cell damage in vitro. ( A ) HK-2 cell viability treated after treatment with different concentrations of ECH. ( B ) HK-2 cell viability under cisplatin stimulation with gradient ECH intervention. ( C ) Representative PI staining images of HK-2 cells and quantitative analysis. ( D ) Western blot and quantitative analysis of KIM-1 and NGAL proteins in HK-2 cells. ( E – H ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in HK-2 cells. ns, no significancant; * p < 0.05, ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group.

    Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

    Techniques: In Vitro, Staining, Analysis, Western Blot, Control

    The effect of AVLE on the kidneys of db/db mice (A) Experimental design (B) Changes in kidney volume across groups (C,D) Blood glucose levels and body weight were measured at different time points (E,F) Changes in kidney weight index across groups (G-I) Serum BUN, SCr, and Cys-C levels in each group (J) The expression of KIM-1 protein was detected by the IHC method. Scale bar = 200 μm (K) Semi-quantitative analysis of KIM-1 protein immunohistochemistry (L,M) KIM-1 protein expression in renal lysates, with β-actin as the internal control. Data are expressed as mean ± SEM (n = 6). Compared with the CON group: ## p < 0.01, ### p < 0.001; compared with the MOD group: * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Apocynum venetum L. leaves extract inhibits ferroptosis and improves renal fibrosis in diabetic kidney disease by regulating the Nrf2/HO-1 pathway

    doi: 10.3389/fphar.2026.1807373

    Figure Lengend Snippet: The effect of AVLE on the kidneys of db/db mice (A) Experimental design (B) Changes in kidney volume across groups (C,D) Blood glucose levels and body weight were measured at different time points (E,F) Changes in kidney weight index across groups (G-I) Serum BUN, SCr, and Cys-C levels in each group (J) The expression of KIM-1 protein was detected by the IHC method. Scale bar = 200 μm (K) Semi-quantitative analysis of KIM-1 protein immunohistochemistry (L,M) KIM-1 protein expression in renal lysates, with β-actin as the internal control. Data are expressed as mean ± SEM (n = 6). Compared with the CON group: ## p < 0.01, ### p < 0.001; compared with the MOD group: * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: KIM-1 rabbit monoclonal antibody , 1:1000 , HUABIO , HA721535.

    Techniques: Expressing, Immunohistochemistry, Control

    Effects of AVLE on HG-induced renal injury and fibrosis-related proteins in HK-2 cells. (A) ZnPP’s impact on the survival of HK-2 cells induced with high glucose. (B,C) Western blot study of each group’s expression levels of the proteins FN, E-cadherin, α-SMA, and KIM-1. All data are presented as mean ± SEM (n = 3). Compared with the NG group: # p < 0.05, ## p < 0.01, ### p < 0.001; compared with the HG group: * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Apocynum venetum L. leaves extract inhibits ferroptosis and improves renal fibrosis in diabetic kidney disease by regulating the Nrf2/HO-1 pathway

    doi: 10.3389/fphar.2026.1807373

    Figure Lengend Snippet: Effects of AVLE on HG-induced renal injury and fibrosis-related proteins in HK-2 cells. (A) ZnPP’s impact on the survival of HK-2 cells induced with high glucose. (B,C) Western blot study of each group’s expression levels of the proteins FN, E-cadherin, α-SMA, and KIM-1. All data are presented as mean ± SEM (n = 3). Compared with the NG group: # p < 0.05, ## p < 0.01, ### p < 0.001; compared with the HG group: * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: KIM-1 rabbit monoclonal antibody , 1:1000 , HUABIO , HA721535.

    Techniques: Western Blot, Expressing

    Macrophage‐Specific Deletion of Zxdb Protects Against Sepsis‐Induced Acute Kidney Injury. (A) Generation strategy for myeloid‐specific Zxdb knockout (Mac‐Zxdb‐KO) mice. (B) H&E staining of kidney sections from Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice. (C‐D) Serum creatinine (Scr) (C) and blood urea nitrogen (BUN) (D) levels in Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice at 24 h post‐CLP. (E) KIM‐1 immunohistochemistry of kidney sections from Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice. (F) TUNEL staining of kidney sections from Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice. (G) Immunofluorescence of kidney sections stained for F480, CD86, and CD206. (H) Serum IL‐1β, IL‐10, and TNF‐α levels in Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice at 24 h post‐CLP. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: The FASEB Journal

    Article Title: ZXDB Drives Macrophage Inflammatory Programming in Sepsis‐Induced Acute Kidney Injury by Recruiting EIF4A3 to Enhance ACACA Translation

    doi: 10.1096/fj.202502962RR

    Figure Lengend Snippet: Macrophage‐Specific Deletion of Zxdb Protects Against Sepsis‐Induced Acute Kidney Injury. (A) Generation strategy for myeloid‐specific Zxdb knockout (Mac‐Zxdb‐KO) mice. (B) H&E staining of kidney sections from Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice. (C‐D) Serum creatinine (Scr) (C) and blood urea nitrogen (BUN) (D) levels in Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice at 24 h post‐CLP. (E) KIM‐1 immunohistochemistry of kidney sections from Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice. (F) TUNEL staining of kidney sections from Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice. (G) Immunofluorescence of kidney sections stained for F480, CD86, and CD206. (H) Serum IL‐1β, IL‐10, and TNF‐α levels in Sham, WT SI‐AKI, and Mac‐Zxdb‐KO SI‐AKI mice at 24 h post‐CLP. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Sections were blocked and incubated overnight at 4°C using primary antibody against KIM‐1 (AF1817; R&D Systems, 1:200).

    Techniques: Knock-Out, Staining, Immunohistochemistry, TUNEL Assay, Immunofluorescence